[tt] Next Big Future - 3 new articles
Eugen Leitl
<eugen at leitl.org> on
Tue Jul 1 06:49:12 UTC 2008
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To: eugen <eugen at leitl.org>
Subject: Next Big Future - 3 new articles
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Here are the latest updates for eugen at leitl.org
"[2]Next Big Future" - 3 new articles
1. [3]Molecular Genetics of Aging conference september 24-28, 2008
2. [4]Electrostatic-based DNA Microarray Technique Could
Revolutionize Medical Diagnostics
3. [5]LIFT cancer clinical trials
4. [6]More Recent Articles
5. [7]Search Next Big Future
[8]Molecular Genetics of Aging conference september 24-28, 2008
The fourth meeting on the Molecular Genetics of Aging is being
organized by Steven Austad, University of Texas Health Science Center;
Judith Campisi, Lawrence Berkeley National Laboratory, Buck Institute
for Age Research and David Sinclair, Harvard Medical School
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Judith Campisi is working on one of the major [15]SENS life extension
projects to deal with too many old cells. ([16]Apoptosens)
Topics and Co-Chairs:
Genetics I
Heidi Tissenbaum (U Mass Worcester MA USA)
Scott Pletcher (Baylor Houston TX USA)
Genomic Stability
Jan Vijg (Buck Institute Novato CA USA)
Elizabeth Blackburn (UC San Francisco CA USA)
Mitochondria / Metabolism
Peter Rabinovitch (U Washington Seattle WA USA)
Leonard Guarente (MIT Boston USA)
Cellular Senescence / Apoptosis / Stress
John Sedivy (Brown U Providence RI USA
Norman Sharpless (UNC Chapel Hill NC USA)
Stem Cells
Irina Conboy (UC Berkeley CA USA)
Karl Rudolph (Med Sch Hanover Germany)
Proliferative Homeostasis
Paul Hasty (UT Health Science Center, San Antonio, TX USA)
Rolf Bodmer (Burnham Institute San Diego CA USA)
Environment / Interventions
Richard Miller (U. Mich., Ann Arbor MI USA)
Steven Spindler (UC Riverside CA USA)
Genetics II
Anne Brunet (Stanford U CA USA)
Jan Hoeijmakers (Erasmus U Rotterdam Netherlands)
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[34]Electrostatic-based DNA Microarray Technique Could Revolutionize Medical
Diagnostics
[35]A team of researchers with the U.S. Department of Energy's
Lawrence Berkeley National Laboratory (Berkeley Lab) has invented a
technique in which DNA or RNA assays -- the key to genetic profiling
and disease detection -- can be read and evaluated without the need of
elaborate chemical labeling or sophisticated instrumentation.
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Based on electrostatic repulsion -- in which objects with the same
electrical charge repel one another -- the technique is relatively
simple and inexpensive to implement, and can be carried out in a
matter of minutes.
"One of the most amazing things about our electrostatic detection
method is that it requires nothing more than the naked eye to read out
results that currently require chemical labeling and confocal laser
scanners," said Jay Groves, a chemist with joint appointments at
Berkeley Lab's Physical Biosciences Division and the Chemistry
Department of the University of California (UC) at Berkeley, who led
this research. "We believe this technique could revolutionize the use
of DNA microarrays for both research and diagnostics."
Groves, who is also a Howard Hughes Medical Institute (HHMI)
investigator, and members of his research group Nathan Clack and
Khalid Salaita, have published a paper on their technique in the
journal Nature Biotechnology, which is now available online. The paper
is entitled "Electrostatic readout of DNA microarrays with charged
microspheres."
In their paper, Groves, Clack, and Salaita describe how dispersing a
fluid containing thousands of electrically-charged microscopic beads
or spheres made of silica (glass) across the surface of a DNA
microarray and then observing the Brownian motion of the spheres
provides measurements of the electrical charges of the DNA molecules.
These measurements can in turn be used to interrogate millions of DNA
sequences at a time. What's more, these measurements can be observed
and recorded with a simple hand-held imaging device -- even a cell
phone camera will do.
"The assumption has been that no detection technique could be more
sensitive than fluorescent labeling, but this is completely untrue, as
our results have plainly demonstrated," said Groves. "We've shown that
changes in surface charge density as a result of specific DNA
hybridization can be detected and quantified with 50-picometer
sensitivity, single base-pair mismatch selectivity, and in the
presence of complex backgrounds. Furthermore, our electrostatic
detection technique should render DNA and RNA microarrays sufficiently
cost effective for broad world-health applications, as well as
research."
FURTHER READING
[42]Nature biotechnology paper: Electrostatic readout of DNA
microarrays with charged microspheres
DNA microarrays are used for gene-expression profiling,
single-nucleotide polymorphism detection and disease diagnosis1, 2,
3. A persistent challenge in this area is the lack of microarray
screening technology suitable for integration into routine clinical
care4, 5. Here, we describe a method for sensitive and label-free
electrostatic readout of DNA or RNA hybridization on microarrays.
The electrostatic properties of the microarray are measured from
the position and motion of charged microspheres randomly dispersed
over the surface. We demonstrate nondestructive electrostatic
imaging with 10-m lateral resolution over centimeter-length scales,
which is four-orders of magnitude larger than that achievable with
conventional scanning electrostatic force microscopy. Changes in
surface charge density as a result of specific hybridization can be
detected and quantified with 50-pM sensitivity, single base-pair
mismatch selectivity and in the presence of complex background.
Because the naked eye is sufficient to read out hybridization, this
approach may facilitate broad application of multiplexed assays
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[59]Rate 'Electrostatic-based DNA Microarray Technique Could
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[60]LIFT cancer clinical trials
[61]Human trials starting for Zheng Cui's LIFT 'cancer cure' H/T
[62]to Alfin
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For the upcoming study, the researchers are currently recruiting
500 local potential donors who are 50 years old or younger and in
good health to have their blood tested. Of those, 100 volunteers
with high cancer-killing activity will be asked to donate white
blood cells for the study. Cell recipients will include 22 cancer
patients who have solid tumors that either didn't respond
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[69]The LIFT method and signing up for the trials The procedure was
previously called GIFT.
LIFT is an investigational new cancer treatment that will transfer
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of a selected donor into the body of a cancer patient.
Here's how the LIFT method works:
* Donor selection: Healthy young volunteers will be screened for the
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* Granulocyte collection: When a qualified patient is identified for
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health impact on granulocyte donation is much smaller than on whole
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* Patient selection and granulocyte infusion: Qualified patients will
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Granulocyte infusion therapy has been traditionally used for treating
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The main focus of the trial is the possibility of developing
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Donor granulocytes per se are not known to produce TA-GVHD. However,
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subjects for this trial.
FURTHER READING
[70]More at redorbit
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68. http://www.feedblitz.com/adfaq.asp
69. http://www.feedblitz.com/t.asp?/64651/2987253/http://www1.wfubmc.edu/LIFT
70. http://www.feedblitz.com/t.asp?/64651/2987253/http://www.redorbit.com/news/health/1455086/cancer_cure_in_mice_to_get_human_trials/
71. http://feeds.feedburner.com/~a/blogspot/advancednano?a=fAPw6r
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----- End forwarded message -----
--
Eugen* Leitl <a href="http://leitl.org">leitl</a> http://leitl.org
______________________________________________________________
ICBM: 48.07100, 11.36820 http://www.ativel.com http://postbiota.org
8B29F6BE: 099D 78BA 2FD3 B014 B08A 7779 75B0 2443 8B29 F6BE
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