[tt] Next Big Future - 3 new articles
Eugen Leitl
<eugen at leitl.org> on
Fri Apr 11 10:16:40 UTC 2008
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Date: Thu, 10 Apr 2008 03:31:03 -0400
To: eugen <eugen at leitl.org>
Subject: Next Big Future - 3 new articles
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Here are the latest updates for eugen at leitl.org
"[2]Next Big Future" - 3 new articles
1. [3]20% of the readers of Nature use cognitive enhancers
2. [4]Regenerative medicine advance: 140 Cell Types From Human
Embryonic Stem Cells
3. [5]Bakken news from around the internet on the eve of the USGS
report
4. [6]More Recent Articles
5. [7]Search Next Big Future
[8]20% of the readers of Nature use cognitive enhancers
[9]20% of the surveyed readers of Nature use cognitive enhancers to
improve their focus, concentration, or memory
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[16]This is related to my article that the transhumanist debate needs
to be properly framed. My general view is that if the negative health
aspects of any form of enhancement can be made less than the benefits
then on an individual basis people will choose enhancements that
personally benefit them.
A total of 1,400 people from 60 countries responded to the online
survey. The subjects were asked specifically about the use of three
drugs: methylphenidate (Ritalin), which is used to treat attention
deficit hyperactivity disorder (ADHD), but is considered on college
campuses as a "study aid"; modafinil (Provigil), which is
prescribed for sleep disorders, but is used by some to fight
general fatigue or jet lag; beta-blockers, cardiovascular drugs
prescribed for heart failure and high blood pressure, which are
also known for their anti-anxiety effect.
Among those "who choose to use," methylphenidate was the most
popular agent: 62 percent of users reported taking it. Modafinil
was taken by 44 percent of users and beta blockers by 15 percent.
Thus, many of the subjects were using more than one drug
When asked about use of other drugs, many of the subjects reported
taking Adderall, a drug prescribed for ADHD containing a mixture of
amphetamines. Other drugs used included centrophenoxine, piracetam,
dextroamphetamine sulfate, and alternative medicines, such as
ginkgo and omega-3 fatty acids.
The use of cognition-enhancing drugs did not vary by age group, the
report indicates. Maher said this may be surprising to some people
since prior research has suggested increased usage in 18- to
25-years-olds.
Improving concentration was the main reason cited for using these
drugs with enhancing focus on a specific task being a close second.
Four fifths of respondents believed that healthy adults should be
permitted to take cognition-enhancing agents if they want to and 69
percent said they would risk mild adverse effects to take the drugs
themselves.
Eighty-six percent of respondents said that children under 16 years
should be restricted from using these drugs, yet one third of
respondents said they would feel pressured to give their child
these agents if other children were taking them.
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[33]Regenerative medicine advance: 140 Cell Types From Human Embryonic Stem
Cells
[34]Research published this week in Regenerative Medicine, reports on
a new technology that yields over 140 previously uncharacterized cell
types, many on an industrial scale. This advance holds great promise
for future research and may one day lead to many new cell-based
therapies in the emerging field of regenerative medicine.
Previously there have been occasional reports that individual cell
types have been generated from hES (human embryonic stem cells) cells,
such cells have often been generated in small quantities, not useful
on an industrial scale.
In a paper titled [35]The ACTCellerate Initiative: large-scale
combinatorial cloning of novel human embryonic stem cell
derivatives", a team led by Dr Michael D. West, now CEO, BioTime,
Inc and Adjunct Professor, University of California, Berkeley,
along with collaborators at Advanced Cell Technology, the Burnham
Institute, Ontario Cancer Institute, and the University of
California, San Francisco demonstrated that primitive precursors of
the many body cell types have an unpredicted ability to be
propagated from a single cell, leading to the clonal expansion of
these embryonic progenitor cell types. A careful genome-wide
analysis of gene expression showed evidence that the "Zip code"
that the developing body uses to place cells in their proper
location in the body is preserved in these cells, giving
researchers a means to make cell types from a single location in
the body.
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Another important finding in this publication is that these highly
purified cell types show that primitive embryonic cell types show
the expression of genes generally associated with malignant cancer.
However, when used in this highly purified form, no malignant
tumors could be observed when the cells were injected into mice.
Dr Chris Mason (UCL), Associate Editor of Regenerative Medicine
said, "This is an enormously exciting development for the regen
sector. The research reported by Dr West and his team represents a
quantum leap forward in embryomics, the mapping and
characterization of the cells of early human development. Without
any doubt, the ACTCellerate technology will greatly hasten the
translation of human embryonic stem cell-based therapies into safe
and effective products for routine clinical practice ".
"The demonstration that combinatorial cloning can lead to numerous
and diverse purified cell types opens the door strategies to map
the human embryome. This roadmap is critical to the clinical
application of the emerging field of regenerative medicine", said
Dr. West.
EXECUTIVE SUMMARY
The unique challenges of pluripotency
o Human embryonic stem (hES) cells show the capacity to
differentiate into all of the hundreds of somatic cell lineages in
the developing human.
o Human embryonic progenitor (hEP) cells are primitive precursors
of terminally differentiated cells that are capable of propagation
in vitro and display makers generally associated with the embryonic
stages of development.
o Challenges to the field include the mapping of the `embryome' for
example to identify the unique molecular markers that allow the
identification and isolation of hEP cells.
Multiplex generation & characterization of hEP cell clones
o The ACTCellerate protocol utilizes a two-step differentiation and
propagation protocol to isolate clonal populations of scalable hEP
cell lines. Of 1090 clones isolated in this report, 280 lines
(25.7%) expanded to at least four roller bottles.
Clonal hEP cells do not display hES markers but instead show
markers of diverse primitive embryonic progenitors
o Cell lines derived with the ACTCellerate protocol do not express
markers of hES cells such as hTERT or OCT4.
o A total of 71% of the hEP cell lines show positive expression for
MEOX1, MEOX2, or FOXF1, which in the mouse are reported to be
expressed only in early stages of embryonic development.
o Non-negative matrix factorization suggested that the complexity
of distinct cell types isolated was at least 140.
Immunocytochemical confirmation of hEP microarray gene-expression
analysis
o Selected markers in putative ectodermal, mesodermal, endodermal
and neural crest gene cell lines were confirmed by
immunocytochemistry.
Clonal hEP lines express diverse cell surface antigens
o Flow cytometry confirmed that gene expression often predicts CD
antigen expression on the cell surface and provides a means of
manipulating hEP cell types.
hEP clones express unique secreted factors
o hEP cell clones expressed diverse secreted growth factors. Select
factors confirmed by ELISA included AREG, FGF7, IGFBP5, TGFb-1 and
PDGF-BB.
hEP cells lack tumorigenicity
o hEP cell lines expressed a wide array of oncofetal genes
including: SILV, PLAG1, AMIGO2, HCLS1, SPINK1, PRAME, INSM1, ENC1
and CEACAM1, as well as others.
o Clonal hEP cell lines did not generate malignant tumors in 4-6
months at doses of 10 million cells per mouse when injected
subcutaneously and intramuscularly.
hEP cells include clones with a robust & mortal proliferative
capacity
o hES cells express high levels of telomerase activity by telomeric
repeat amplification protocol (TRAP) assay, and an immortal
phenotype when maintained in the undifferentiated state.
o hEP cells were telomerase negative by TRAP assay, but often
display a long proliferative lifespan in vitro useful in scaling
the cells.
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[58]Bakken news from around the internet on the eve of the USGS report
My guess at what the USGS will report as the recoverable oil in the
Bakken formation tomorrow.
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[65]Stackfrac horizontal drilling that is available today could
recover up to 15% of the oil in parts of the formation. 75% of the
formation is in the USA. 25% is in Canada. Not all parts of the
formation would have 15% recovery, some might have 0-10%. Even though
future technology could improve this number, that is not what will be
projected. The high price of oil means that quick wells that produce
for as little as 6-12 months could be profitably drilled.
I will say for the US portion 380 billion barrels of oil in place and
42 billion barrels of recoverable oil with todays technology. My guess
is whole formation has 510 billion barrels of oil in place. 15 Billion
barrels of recoverable oil in Canada.
[66]Business Week reports on the Bakken and the imminent USGS report
[67]Salon has brief coverage.
[68]Bakken shale blog is of course solely focused on the Bakken
[69]The Cost of Energy summarizes some of the data and reports and
agrees there is a lot of oil in place but wants the USGS and other
sources to pin down whether recoverable oil is 3% or 50% and then will
not say how fast it will be developed
[70]Classical values feels that the Bakken shows that capitalism beats
fear mongers again
[71]Resource Investor has a news roundup on Bakken
[72]North Dakota Press release from 2006
FURTHER READING
[73]USGS 1995 study and current decline rates
[74]Estimates of bakken oil size and timing
[75]Multistage fracturing horizontal drilling technique
[76]Bakken oil not fully valued
[77]Bakken oil is highly profitable
[78]A Saudi Arabia of oil in Bakken and Toquay formations in the USA
and Canada
[79]More bakken news from Google news
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----- End forwarded message -----
--
Eugen* Leitl <a href="http://leitl.org">leitl</a> http://leitl.org
______________________________________________________________
ICBM: 48.07100, 11.36820 http://www.ativel.com http://postbiota.org
8B29F6BE: 099D 78BA 2FD3 B014 B08A 7779 75B0 2443 8B29 F6BE
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